A family of small tyrosine rich proteins is essential for oogonial and oospore cell wall development of the mycoparasitic oomycete Pythium oligandrum.
Identifieur interne : 001397 ( Main/Exploration ); précédent : 001396; suivant : 001398A family of small tyrosine rich proteins is essential for oogonial and oospore cell wall development of the mycoparasitic oomycete Pythium oligandrum.
Auteurs : Laura J. Grenville-Briggs [Suède] ; Neil R. Horner ; Andrew J. Phillips ; Gordon W. Beakes ; Pieter Van WestSource :
- Fungal biology [ 1878-6146 ] ; 2013.
Descripteurs français
- KwdFr :
- Famille multigénique (MeSH), Maladies des plantes (parasitologie), Paroi cellulaire (génétique), Paroi cellulaire (métabolisme), Protéines (génétique), Protéines (métabolisme), Pythium (croissance et développement), Pythium (génétique), Pythium (métabolisme), Spores (croissance et développement), Spores (génétique), Spores (métabolisme), Transport des protéines (MeSH).
- MESH :
- croissance et développement : Pythium, Spores.
- génétique : Paroi cellulaire, Protéines, Pythium, Spores.
- métabolisme : Paroi cellulaire, Protéines, Pythium, Spores.
- parasitologie : Maladies des plantes.
- Famille multigénique, Transport des protéines.
English descriptors
- KwdEn :
- Cell Wall (genetics), Cell Wall (metabolism), Multigene Family (MeSH), Plant Diseases (parasitology), Protein Transport (MeSH), Proteins (genetics), Proteins (metabolism), Pythium (genetics), Pythium (growth & development), Pythium (metabolism), Spores (genetics), Spores (growth & development), Spores (metabolism).
- MESH :
- chemical , genetics : Proteins.
- genetics : Cell Wall, Pythium, Spores.
- growth & development : Pythium, Spores.
- metabolism : Cell Wall, Proteins, Pythium, Spores.
- parasitology : Plant Diseases.
- Multigene Family, Protein Transport.
Abstract
The mycoparasitic oomycete Pythium oligandrum is homothallic, producing an abundance of thick-walled spiny oospores in culture. After mining a cDNA sequence dataset, we identified a family of genes that code for small tyrosine rich (Pythium oligandrumsmall tyrosine rich (PoStr)) proteins. Sequence analysis identified similarity between the PoStr proteins and putative glycine-rich cell wall proteins from the related plant pathogenic oomycete Pythium ultimum, and mating-induced genes from the oomycete Phytophthora infestans. Expression analysis showed that PoStr transcripts accumulate during oospore production in culture and immunolocalisation indicates the presence of these proteins in oogonial and oospore cell walls. PoStr protein abundance correlated positively with production of oogonia as determined by antibiotic-mediated oogonia suppression. To further characterise the role of PoStr proteins in P. oligandrum oospore production, we silenced this gene family using homology-dependent gene silencing. This represents the first characterisation of genes using gene silencing in a Pythium species. Oospores from silenced strains displayed major ultrastructural changes and were sensitive to degradative enzyme treatment. Oogonia of silenced strains either appeared to be arrested at the mature oosphere stage of development or in around 40 % of the structures, showed a complete suppression of oospore formation. Suppressed oogonia were highly vacuolated and the oogonium wall was thickened by a new inner wall layer. Our data suggest PoStr proteins are probably integral structural components of the normal oospore cell wall and play a key role in oospore formation.
DOI: 10.1016/j.funbio.2013.01.001
PubMed: 23537873
Affiliations:
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Grenville-Briggs</name><affiliation wicri:level="1"><nlm:affiliation>Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, Stockholm SE-106 91, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, Stockholm SE-106 91</wicri:regionArea><wicri:noRegion>Stockholm SE-106 91</wicri:noRegion></affiliation></author><author><name sortKey="Horner, Neil R" sort="Horner, Neil R" uniqKey="Horner N" first="Neil R" last="Horner">Neil R. Horner</name></author><author><name sortKey="Phillips, Andrew J" sort="Phillips, Andrew J" uniqKey="Phillips A" first="Andrew J" last="Phillips">Andrew J. Phillips</name></author><author><name sortKey="Beakes, Gordon W" sort="Beakes, Gordon W" uniqKey="Beakes G" first="Gordon W" last="Beakes">Gordon W. Beakes</name></author><author><name sortKey="Van West, Pieter" sort="Van West, Pieter" uniqKey="Van West P" first="Pieter" last="Van West">Pieter Van West</name></author></analytic><series><title level="j">Fungal biology</title><idno type="ISSN">1878-6146</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell Wall (genetics)</term><term>Cell Wall (metabolism)</term><term>Multigene Family (MeSH)</term><term>Plant Diseases (parasitology)</term><term>Protein Transport (MeSH)</term><term>Proteins (genetics)</term><term>Proteins (metabolism)</term><term>Pythium (genetics)</term><term>Pythium (growth & development)</term><term>Pythium (metabolism)</term><term>Spores (genetics)</term><term>Spores (growth & development)</term><term>Spores (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Famille multigénique (MeSH)</term><term>Maladies des plantes (parasitologie)</term><term>Paroi cellulaire (génétique)</term><term>Paroi cellulaire (métabolisme)</term><term>Protéines (génétique)</term><term>Protéines (métabolisme)</term><term>Pythium (croissance et développement)</term><term>Pythium (génétique)</term><term>Pythium (métabolisme)</term><term>Spores (croissance et développement)</term><term>Spores (génétique)</term><term>Spores (métabolisme)</term><term>Transport des protéines (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Proteins</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Pythium</term><term>Spores</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cell Wall</term><term>Pythium</term><term>Spores</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Pythium</term><term>Spores</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Paroi cellulaire</term><term>Protéines</term><term>Pythium</term><term>Spores</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term><term>Proteins</term><term>Pythium</term><term>Spores</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Paroi cellulaire</term><term>Protéines</term><term>Pythium</term><term>Spores</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Maladies des plantes</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Plant Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Multigene Family</term><term>Protein Transport</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Famille multigénique</term><term>Transport des protéines</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The mycoparasitic oomycete Pythium oligandrum is homothallic, producing an abundance of thick-walled spiny oospores in culture. After mining a cDNA sequence dataset, we identified a family of genes that code for small tyrosine rich (Pythium oligandrumsmall tyrosine rich (PoStr)) proteins. Sequence analysis identified similarity between the PoStr proteins and putative glycine-rich cell wall proteins from the related plant pathogenic oomycete Pythium ultimum, and mating-induced genes from the oomycete Phytophthora infestans. Expression analysis showed that PoStr transcripts accumulate during oospore production in culture and immunolocalisation indicates the presence of these proteins in oogonial and oospore cell walls. PoStr protein abundance correlated positively with production of oogonia as determined by antibiotic-mediated oogonia suppression. To further characterise the role of PoStr proteins in P. oligandrum oospore production, we silenced this gene family using homology-dependent gene silencing. This represents the first characterisation of genes using gene silencing in a Pythium species. Oospores from silenced strains displayed major ultrastructural changes and were sensitive to degradative enzyme treatment. Oogonia of silenced strains either appeared to be arrested at the mature oosphere stage of development or in around 40 % of the structures, showed a complete suppression of oospore formation. Suppressed oogonia were highly vacuolated and the oogonium wall was thickened by a new inner wall layer. Our data suggest PoStr proteins are probably integral structural components of the normal oospore cell wall and play a key role in oospore formation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23537873</PMID><DateCompleted><Year>2013</Year><Month>09</Month><Day>03</Day></DateCompleted><DateRevised><Year>2019</Year><Month>10</Month><Day>08</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1878-6146</ISSN><JournalIssue CitedMedium="Internet"><Volume>117</Volume><Issue>3</Issue><PubDate><Year>2013</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Fungal biology</Title><ISOAbbreviation>Fungal Biol</ISOAbbreviation></Journal><ArticleTitle>A family of small tyrosine rich proteins is essential for oogonial and oospore cell wall development of the mycoparasitic oomycete Pythium oligandrum.</ArticleTitle><Pagination><MedlinePgn>163-72</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.funbio.2013.01.001</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S1878-6146(13)00002-0</ELocationID><Abstract><AbstractText>The mycoparasitic oomycete Pythium oligandrum is homothallic, producing an abundance of thick-walled spiny oospores in culture. After mining a cDNA sequence dataset, we identified a family of genes that code for small tyrosine rich (Pythium oligandrumsmall tyrosine rich (PoStr)) proteins. Sequence analysis identified similarity between the PoStr proteins and putative glycine-rich cell wall proteins from the related plant pathogenic oomycete Pythium ultimum, and mating-induced genes from the oomycete Phytophthora infestans. Expression analysis showed that PoStr transcripts accumulate during oospore production in culture and immunolocalisation indicates the presence of these proteins in oogonial and oospore cell walls. PoStr protein abundance correlated positively with production of oogonia as determined by antibiotic-mediated oogonia suppression. To further characterise the role of PoStr proteins in P. oligandrum oospore production, we silenced this gene family using homology-dependent gene silencing. This represents the first characterisation of genes using gene silencing in a Pythium species. Oospores from silenced strains displayed major ultrastructural changes and were sensitive to degradative enzyme treatment. Oogonia of silenced strains either appeared to be arrested at the mature oosphere stage of development or in around 40 % of the structures, showed a complete suppression of oospore formation. Suppressed oogonia were highly vacuolated and the oogonium wall was thickened by a new inner wall layer. Our data suggest PoStr proteins are probably integral structural components of the normal oospore cell wall and play a key role in oospore formation.</AbstractText><CopyrightInformation>Copyright © 2013 The British Mycological Society. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Grenville-Briggs</LastName><ForeName>Laura J</ForeName><Initials>LJ</Initials><AffiliationInfo><Affiliation>Division of Glycoscience, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, Stockholm SE-106 91, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Horner</LastName><ForeName>Neil R</ForeName><Initials>NR</Initials></Author><Author ValidYN="Y"><LastName>Phillips</LastName><ForeName>Andrew J</ForeName><Initials>AJ</Initials></Author><Author ValidYN="Y"><LastName>Beakes</LastName><ForeName>Gordon W</ForeName><Initials>GW</Initials></Author><Author ValidYN="Y"><LastName>van West</LastName><ForeName>Pieter</ForeName><Initials>P</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>BB/G012075/1</GrantID><Agency>Biotechnology and Biological Sciences Research Council</Agency><Country>United Kingdom</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>01</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Fungal Biol</MedlineTA><NlmUniqueID>101524465</NlmUniqueID></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011506">Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005810" MajorTopicYN="Y">Multigene Family</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021381" MajorTopicYN="N">Protein Transport</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011506" MajorTopicYN="N">Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011775" MajorTopicYN="N">Pythium</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013170" MajorTopicYN="N">Spores</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>08</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2012</Year><Month>12</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>01</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>3</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>3</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>9</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23537873</ArticleId><ArticleId IdType="pii">S1878-6146(13)00002-0</ArticleId><ArticleId IdType="doi">10.1016/j.funbio.2013.01.001</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Suède</li></country></list><tree><noCountry><name sortKey="Beakes, Gordon W" sort="Beakes, Gordon W" uniqKey="Beakes G" first="Gordon W" last="Beakes">Gordon W. Beakes</name><name sortKey="Horner, Neil R" sort="Horner, Neil R" uniqKey="Horner N" first="Neil R" last="Horner">Neil R. Horner</name><name sortKey="Phillips, Andrew J" sort="Phillips, Andrew J" uniqKey="Phillips A" first="Andrew J" last="Phillips">Andrew J. Phillips</name><name sortKey="Van West, Pieter" sort="Van West, Pieter" uniqKey="Van West P" first="Pieter" last="Van West">Pieter Van West</name></noCountry><country name="Suède"><noRegion><name sortKey="Grenville Briggs, Laura J" sort="Grenville Briggs, Laura J" uniqKey="Grenville Briggs L" first="Laura J" last="Grenville-Briggs">Laura J. Grenville-Briggs</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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